Fig. 2.

Impacts of cell culture medium on zebrafish development. Each circle in the figure represents one animal with an adverse outcome for the indicated endpoint, with statistically adverse outcomes (n = 32; p < 0.05) shown in red. “MO24” describes any observed mortality effects observed at 24 hpf. “MORT” represents any observed mortality effects observed at 120 hpf. “any.effect” represents any adverse effect observed including morphological and mortality at 120 hpf. (A) Embryonic exposures to 0, 20, 40, 60, 80, or 100 % RPMI with 1 % UP and AB. No significant effects were observed at 80 % RPMI with antibiotics at 1 %. (B) Embryonic exposures to FBS solution made of 0, 2, 4, 6, 8, or 10 % FBS with 1 % AB. (C) Embryonic exposures to various combinations of 80 % RPMI, 2 % FBS, and 1 % UP and AB.