Fig. 3.

Embryonic zebrafish responses to ENMs in cell culture medium. Embryonic zebrafish were exposed to ENMs GO 400 nm x 400 nm, GO 1μm x 1 μm, CeO₂, and ZnO where n = 32. Like Fig. 2, each circle represents an adverse outcome, with statistically significant outcomes (p-value < 0.05) shown in red. “MO24” is mortality at 24 hpf. “MORT” is mortality at 120 hpf. “any.effect” is cumulative morphology and mortality at 120 hpf. Fig. 3A, C, E, and G (left side of figure) show effects of ENMs in UP water with no addition of cell culture medium (-). Fig. 3B, D, F, and H (right side of figure) show effects of ENM exposures in the presence of cell culture medium. Nanoparticle concentrations used were 0, 5, 10.7, 23.2, and 50 µg/mL.