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Characterization of CrLNPs. A Cleavage activities of Cas9 stored at various pHs, by adding the RNP with target DNA (target: EGFR). B Hydrodynamic size, C zeta potential, and D cryo-TEM of CrLNPs prepared at different pHs (pH 6.0 and 7.4). Scale bar, 100 nm. E TNS assay of CrLNP prepared at pH 6.0. F Loading efficiency of Cas9 and sgRNA in CrLNPs prepared at different pHs (pH 6.0 and 7.4)
