Table 3.
Oligonucleotide primers used in the current study
Name | 5ʹ → 3ʹ sequence | Tm | Target | Design |
---|---|---|---|---|
psbAu | GTTATGCATGAACGTAATGCTC | 61.0 °C | psbA-trnH intergenic spacer | Sang et al. 70 |
trnHu | CGCGCATGGTGGATTCACAATCC | 76.0 °C | Tate et al. 71 | |
ycf1b_fwu | TCTCGACGAAAATCAGATTGTTG | 69.9 °C | ycf1b coding region | Dong et al. 39 |
yfc1b_rvu | ATACATGTCCAAAGTGATGGAAA | 59,8 °C | ||
ycf1b_Mo_ ARMS_rvd | CTTTGATCGTTGGGATCAGTCAG | 66.7 °C | diagnostic SNP in M oleifera ycf1b | current study |
ycf1b_NoMo_ ARMS_rvd | GTTTGAATTTTCTTGAACGAATCTG | 63,6 °C | diagnostic SNP in Moringa species other than M. oleifera | current study |
u universal.
d diagnostic.