Fig. 4.

Visualization of alternative splicing isoforms of differential alternative splicing (DAS) gene MSTRG.36906 under cold and salinity stress. Sashimi plots depict differential alternative splicing (DAS) through exon skipping (SE) events of gene MSTRG.36906 in response to different abiotic stressors. Individual plots for control (mock, gray), cold stress (blue), and salinity stress (pink) visualize the read coverage and junction reads which are plotted as arcs with the indicated junction read counts. Below each plot alternative splicing isoforms are visualized in dark blue. The Benjamini–Hochberg method was applied to obtain p-values corrected for false-discovery rate (FDR), and DAS events were identified using a cutoff of |ΔIncLevel| ≥ 0.1 and FDR < 0.05.