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. Author manuscript; available in PMC: 2024 Apr 14.
Published in final edited form as: J Invest Dermatol. 2003 Jan;120(1):116–122. doi: 10.1046/j.1523-1747.2003.12012.x

Figure 1. IL-12 inhibits UVB-induced TNFα production by cell.

Figure 1

(a) IL-12 inhibits UVB-induced neonatal keratinocyte TNFα production. Neonatal keratinocytes were irradiated with 30 mJ per cm2 of UVB, 5 J per cm2 of UVA, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-12 per ml, followed by 24 h of incubation at 37°C. Displayed are TNFα concentrations in the conditioned media. The TNF ELISA is sensitive to < 2 pg per ml. (b) IL-12 inhibits UVB-induced fibroblast TNFα production. Fibroblasts were irradiated with 30 mJ per cm2 of UVB, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, 10 ng IL-12 per ml, followed by 24 h of incubation at 37°C. Displayed are TNFα concentrations in the conditioned media. (c) IL-12 inhibits UVB-induced adult keratinocyte TNFα production in a dose-dependent manner. Adult keratinocytes were irradiated with 30 mJ per cm2 of UVB, 5 J per cm2 of UVA, or sham irradiated, and then given media with 0–5000 pg IL-12 per ml, followed by 24 h of incubation at 37°C. Displayed are TNFα concentrations in the conditioned media.