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Quantitative comparison of collagenase message levels. RNA was extracted from cultured control fibroblast monolayers (lane 1) 24 h after IL-1α (lanes 2–4), ±IL-12 (500 pg per ml) (lane 3), ±anti-TNFα antibody (lane 4), and analyzed by northern blot. The filters were hybridized sequentially with a collagenase cDNA probe and then rehybridized with the 18S probe.
