TABLE 3.
Summary of the results of CHO/HPRT gene mutation assay on Symbiota® (MS‐20).
| Treatment (μL/mL) | S9 (−/+) | Cytotoxicity (%) | Mutation frequency (per 106 clonable cells) |
|---|---|---|---|
| 0 | − | 0 | 0 |
| 3.125 | − | 8.5 | 11.3 |
| 6.25 | − | 14.9 | 5.5 |
| 12.5 | − | 16.2 | 4.1 |
| 25 | − | 17.9 | 9.5 |
| 50 | − | 83.2 | 7.3 |
| 100 | − | 100 | NA |
| Positive a | − | 6.6 | 97.2 |
| 0 | + | 0 | 7.6 |
| 1.5625 | + | 12.6 | 11.3 |
| 3.125 | + | 18.9 | 3.7 |
| 6.25 | + | 12 | 11 |
| 12.5 | + | 27.8 | 16 |
| 25 | + | 45.6 | 26.2 |
| 50 | + | 80.8 | 16.6 |
| Positive b | + | 44 | 125.3 |
a
Positive control: Methanesulfonic acid, ethyl ester (EMS) at 0.1 μ1/ml.
b
Positive control: N‐nitroso‐dimethylamine (DMN) at 100 μg/mL.