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. 2024 Jan 24;31(4):562–573. doi: 10.1038/s41417-024-00731-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The intervention effect of RanGAP1 knockdown and RanGAP1 overexpressed in CRC cells. B–E CCK8 assays and colony formation assays showed the proliferation ability of silencing and overexpressing RanGAP1 on CRC cells. F, G Flow cytometry assays showed the effects of silencing and overexpressing RanGAP1 on the cycle of CRC cells. H, I Injection of modified RanGAP1 knockdown DLD1 cells in nude mice. J Volume of tumors were recorded every four days. K Xenografts from nude mice were measured. L Weight of tumors in each group. M The proteins levels of RanGAP1 and ki67 were quantified.