. 2024 Mar 31;66(2):295–309. doi: 10.5187/jast.2023.e13

Table 3. Species and genus specific primers used for real time PCR to profile selected bacteria.

Target organisms	Primers	Sequence (5'-3')	Product size(bp)	Annealing temperature (°C)	Reference
Total bacteria	Total-F	GTGSTGCAYGGYYGTCGTCA	123	60	Suzuki et al. [45]
Total bacteria	Total-R	ACGTCRTCCMCNCCTTCCTC	123	60	Suzuki et al. [45]
Clostridium cluster IV	C.leptum-F	GCACAAGCAGTGGAGT	240	60	Matsuki et al. [46]
Clostridium cluster IV	C.leptum-R	CTTCCTCCGTTTTGTCAA	240	60	Matsuki et al. [46]
Clostridium clusterXIVa	Clo14-F	CGGTACCTGACTAAGAAGC	189	60	Matsuki et al. [46]
Clostridium clusterXIVa	Clo14-R	AGTTTYATTCTTGCGAACG	189	60	Matsuki et al. [46]
Lactobacillus	Lac-F	AGCAGTAGGGAATCTTCCA	341	64	Khafipour et al. [47]
Lactobacillus	Lac-R	ATTCCACCGCTACACATG	341	64	Khafipour et al. [47]
Bacteroidesspp.	Bac303-F	GAAGGTCCCCCACATTG	126	60	Bartosch et al. [48]
Bacteroidesspp.	Bfr-Fmrev-R	CGCKACTTGGCTGGTTCAG	126	60	Bartosch et al. [48]
Escherichia coli	E.coli-F	CATGCCGCGTGTATGAAGAA	95	60.8	Huijsdens et al. [49]
Escherichia coli	E.coli-R	CGGGTAACGTCAATGAGCAAA	95	60.8	Huijsdens et al. [49]

PCR, polymerase chain reaction.