REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Sox 2 (D9B8N) Rabbit mAb	Cell Signaling Technology	Cat# 23064, RRID: AB_2714146
Oct – 4A (C52G3) Rabbit mAb	Cell Signaling Technology	Cat# 2890, RRID: AB_2167725
NCAM1 (CD56) (123C3) Mouse mAb	Cell Signaling Technology	Cat# 3576, RRID: AB_2149540
Doublecortin Antibody Rabbit polyclonal antibody	Cell Signaling Technology	Cat# 4604, RRID: AB_561007
Anti-Collagen Type II Antibody, clone 6B3	Millipore Sigma	Cat# MAB8880, RRID: AB_2260779
COL1A1 (E6A8E) Rabbit mAb	Cell Signaling Technology	Cat# 39952, RRID: AB_2895124
Recombinant Anti-Elastin antibody (EPR20603)	Abcam	Cat# ab213720
COMP Polyclonal Antibody	ThermoFisher	Cat# PA5-95547, RRID: AB_2807349
Human Matrilin-1 Antibody (Mouse)	R&D Systems	Cat# MAB7878; RRID: AB_3096006
PTPRZ1 (E7C8Z) Rabbit mAb	Cell Signaling Technology	Cat# 15641; RRID: AB_3096003
DDR1 (D1G6) XP Rabbit mAb	Cell Signaling Technology	Cat# 5583, RRID: AB_10694842
Anti-DDR2 antibody (3B11E4)	Abcam	Cat# ab63337, RRID: AB_955827
Sox9 (D8G8H) Rabbit mAB	Cell Signaling Technology	Cat# 82630, RRID: AB_2665492
PDGF Receptor alpha (D1E1E XP Rabbit mAb	Cell Signaling Technology	Cat# 3174, RRID: AB_2162345
Tenascin C (E5J3B) Rabbit mAb	Cell Signaling Technology	Cat# 33352; RRID: AB_3096005
Anti-Decorin antibody (Rabbit)	Abcam	Cat#175404, RRID: Ab_2890261
Prolong Gold Antifade Reagent with DAPI	Cell Signaling Technology	Cat# 8961
Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) raised in goat	Cell Signaling Technology	Cat# 8890, RRID: AB_2714182
Anti-Rabbit IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 488 Conjugate) raised in goat	Cell Signaling Technology	Cat# 4412, RRID: AB_1904025
PE anti-human CD271 (NGFR)	Biolegend	Cat# 345105, RRID: AB_2282827
Chemicals, peptides, and recombinant proteins
Chroman - 1	MedChem Express	Cat# HY-15392, CAS: 1273579-40-0
trans-ISRIB	Tocris	Cat# 5284, CAS: 1597403-47-8
Emricasan (IDN-6556)	Selleckchem	Cat# S7775, CAS: 254750-02-2
Polyamine Supplement (1000X)	Millipore Sigma	P8483
Antioxidant Supplement (1000X)	Millipore Sigma	A1345
CHIR99021	Millipore Sigma	SML1046, CAS: 252917-06-9
SB 431542 hydrate	Millipore Sigma	S4317, CAS: 301836-41-9
SB 431542	Tocris	Cat# 1614, CAS: 301836-41-9
LDN193189	VWR	Cat# 1995-5, 25, CAS: 1062368-24-4
Poly – L -ornithine hydrobromide	Millipore Sigma	P3655, CAS: 27378-49-0
Laminin Mouse Protein, Natural	ThermoFisher	Cat# 23017015,
Fibronectin, Human	Corning	Cat# 356008
Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free	Corning	Cat# 354230
L- Glutamine	ThermoFisher	Cat# 25030081
MEM Non-Essential Amino Acids Solution	ThermoFisher	Cat# 11140050
Β – Mercaptoethanol	ThermoFisher	Cat# 21985023
Insulin from bovine pancreas	Millipore Sigma	I6634, CAS: 11070-73-8
apo – Transferrin Human	Millipore Sigma	T1147, CAS: 11096-37-0
Sodium selenite	Millipore Sigma	S9133, CAS: 10102-18-8
Putrescine dihydrochloride	Millipore Sigma	P5780, CAS: 333-93-7
Progesterone	Millipore Sigma	P6149, CAS: 57-83-0
(+) – Sodium L-ascorbate	Millipore Sigma	A4034, CAS: 134-03-2
Human Brain-Derived Neurotrophic Factor (BDNF) Recombinant Protein	Cell Signaling Technology	Cat# 3897, UniProt ID: #P23560
Recombinant Human/Murine FGF-8b	Peprotech	Cat# 100-25, UniProt ID: P55075
Recombinant Murine Sonic Hedgehog (Shh)	Peprotech	Cat# 315-22, UniProt ID: Q62226
Y- 27632 (Dihydrochloride)	Stemcell Technologies	Cat# 72304, CAS: 129830-38-2
Recombinant Human FGF-basic	Peprotech	Cat# 100-18b, UniProt ID: P09038
Human Epidermal Growth Factor (hEGF)	Cell Signaling Technology	Cat# 8916, UniProt ID: P01133
Deposited data
snRNA-seq	Mendeley Data	https://data.mendeley.com/datasets/49gxpjrcn9/1
Proteomic data	PRIDE	http://www.ebi.ac.uk/pride/archive/projects/PXD050668
Networks (CFN and CFN plus CCCN)	NDEx	https://www.ndexbio.org/viewer/networks/78c8f03d-d592-11ee-8a40-005056ae23aa https://www.ndexbio.org/viewer/networks/fa2c707c-d653-11ee-8a40-005056ae23aa
Experimental models: Cell lines
WA09/H9 Human	WiCell	RRID: CVCL_9773
H20961 iPS Cells	Weizmann Institute of Science	RRID: CVCL_HA53
Software and algorithms
Seurat	CRAN	RRID: SCR_007322
Connectome	GitHub	https://github.com/msraredon/Connectome
RCy3	Bioconductor	RRID: SCR_006442
enrichR	CRAN	RRID: SCR_001575
Other
Robosep Buffer 2	Stemcell Technologies	Cat# 20164
EasySep Magnet	Stemcell Technologies	Cat# 18000
EasySep Release Human PSC-derived Neural Crest Cell Positive Selection Kit	Stemcell Technologies	Cat# 100-0047
Anti-FITC Microbeads	Miltenyi Biotec	Cat# 130-048-܁701
MACS Multistand	Miltenyi Biotec	Cat# 130-042-303
MS Columns	Miltenyi Biotec	Cat# 130-042-201
DMEM F-12 Media	ThermoFisher	Cat# 12500-096
mTeSR1 Media	StemcellTechnologies	Cat# 85850
mTeSR1 Plus Media	StemcellTechnologies	Cat# 100-0483
ReLeSR	StemcellTechnologies	Cat# 100-0276
Knockout DMEM	ThermoFisher	Cat# 10829018
Knockout Serum Replacement	ThermoFisher	Cat# 108280