Expression levels of Cx43 in LPS-induced macrophage polarization. (A) Total protein levels of Cx43 in RAW264.7 macrophages in the control and LPS groups. (B) Statistical analysis of Cx43. Data are presented as the mean ± SEM (n = 3). *P < .05 vs control groups. (C) Expression levels of Cx43 in RAW264.7 macrophages as detected by immunofluorescence (n = 3). (D) Statistical analysis of Cx43. Data are presented as the mean ± SEM (n = 3). *P < .05 vs control groups. (E) Total protein levels of Cx43 in RAW264.7 macrophages in the control, Gap26 and Gap19 groups. (F) Statistical analysis of Cx43. Data are presented as the mean ± SEM (n = 3). *P < .05 vs control groups. #P < .05 vs LPS groups. (G–H) The total expression levels of CD86 in RAW264.7 macrophages in the control, LPS, LPS + Gap19, and LPS + Gap26 groups (n = 3). (I–J) The total expression level of iNOS in RAW264.7 macrophages in the control, LPS, LPS + Gap19, and LPS + Gap26 groups (n = 3). Data are presented as the mean ± SEM (n = 3). *P < .05 vs control groups. #P < .05 vs LPS groups. Cx43 = connexin 43, iNOS = inducible nitric oxide synthase, LPS = lipopolysaccharide, PI = propidium iodide.