Table 1. ELISA IgG reactivity against recombinant Pf77 and PfMDV-1 in serum samples collected from Ghanaian adults.

ELISA IgG reactivity was determined in serum samples (n = 100) collected from adults living in a malaria endemic area in the Greater Accra Region of Ghana. Serum samples (n = 5) from U.S. residents who had never been exposed to malaria served as negative controls. Reactivity was determined by comparison of the average optical density (OD)RR₄₁₀RR of each sample, run in triplicate, and the ODRR₄₁₀RR of the negative control wells. All serum samples were tested at 1:100 dilution. The threshold for positivity was set at the mean ODRR₄₁₀RR of the negative control wells + 2 SDs.

	Pf77 (n = 100)	PfMDV-1 (n = 100)
Proportion reactive	52%	90%
Mean OD410 of reactive samples	2.19 ± 0.34*	1.86 ± 0.66*

^*The mean OD₄₁₀ among samples reactive to recombinant Pf77 was significantly higher than that of samples reactive to recombinant PfMDV-1 (P = 0.001).