Table 1. The primer sequences were used in the detection of cytokine expression and IBV using real-time machine.
| Gene | Primer sequence | Reference |
|---|---|---|
| Cytokine expression genes | ||
| GAPDH | F-TGCTGCCCAGAACATCATCC R-ACGGCAGGTCAGGTCAACAA |
Huang et al. (2021) |
| IL-1β | F-GCTCTACATGTCGTGTGTGATGAG R-TGTCGATGTCCCGCATGA |
|
| IFN-γ | F-ATCATACTGAGCCAGATTGTTTCG R-TCTTTCACCTTCTTCACGCCAT |
|
| IFN-β | F: ACA CTG ACA AGT CAA AGC CGC ACA R: AGT CGT TCA TCG GGA GCT TGG C |
Villanueva et al. (2011) |
| OAS | F: AGA ACT GCA GAA GAA CTT TGT C R: GCT TCA ACA TCT CCT TGT ACC |
|
| IBV-specific primers and probes targeting a fragment of the nucleocapsid (N) gene | ||
| IBV-pan FW-1 | CAG TCC CDG ATG CNT GGT A | Naguib et al. (2017) |
| IBV-pan FW-2 | CAG TCC CDG ACG CGT GGT A | |
| IBV-pan RV | CC TTW SCA GMA ACM CAC ACT | |
| IBV-pan Probe | ACTGGA ACA GGA CCD GCC GCT GAC CT | |