Table 1.
Summary of G-subunit homolog search results
| Method | Query | Database | Total hits (including canonical G-subunit proteins) | Distant hits | E-value range of distant hits | TM-scorea of distant hits | Annotation of distant hits | Taxonomy of distant hits |
|---|---|---|---|---|---|---|---|---|
| BLASTP | A. vinelandii VnfG | NCBI nonredundant proteins (nr) | 433 | 0 | n/a | n/a | n/a | n/a |
| TBLASTN | A. vinelandii VnfG | NCBI nucleotide collection (nr/nt) | 205 | 0 | n/a | n/a | n/a | n/a |
| PSI-BLAST | A. vinelandii VnfG | NCBI nonredundant proteins (nr) | 446 | 7 | 0.026 to 0.064 | n/a | TIGR04141 family sporadically distributed protein; Uncharacterized protein | Pseudomonas spp. |
| PSI-BLAST | VnfAnfAnc G | NCBI nonredundant proteins (nr) | 543 | 0 | n/a | n/a | n/a | n/a |
| HMMER hmmsearch | Extant G-subunit alignment | UniProt Reference Proteomes | 94 | 1 | 0.061 | n/a | Uncharacterized protein | Coregonus sp. |
| HMMER hmmsearch | Extant & ancestral G-subunit alignment | UniProt Reference Proteomes | 96 | 3 | 4.0e−09 to 0.01 | n/a | Uncharacterized protein | Brugia malayi, Loa loa |
| FoldSeek | A. vinelandii VnfG (PDB 5N6Y) | Protein Data Bank | 3 | 0 | n/a | n/a | n/a | n/a |
| FoldSeek | A. vinelandii VnfG (PDB 5N6Y) | AlphaFold-UniProt/Swiss-Prot | 39 | 2 | 5.2 to 8.7 | 0.52 to 0.54 | Uncharacterized protein | Vibrio vulnificus, Deinococcus grandis |
aTemplate modeling score for structural alignments.