Fig. 1.

Identification of immunological subtypes based on TIL-Bs by single cell analysis. a UMAP of tumor infiltrating immune cells (n = 105.943 cells) from 26 samples in HNSCC scRNA-seq, colored by cell clusters. b UMAP plots showing expression of classical marker genes from immune cell clusters. c Heatmap of signature genes for immune cell clusters. Each cell cluster is represented by five specifically expressed genes. d Bar plot showing the immune cell type proportion in each HNSCC sample. e UMAP plot showing 15 B cells clusters. f Two subgroups were identified using ConsensusClusterPlus R package based on the marker genes. g t-SNE plot of HNSCC scRNA-seq cohort (n = 26), colored by sample groups