Fig. 3.

Validation of classification method based on TIL-Bs in another dataset. a Two subgroups were identified using ConsensusClusterPlus R package based on the B-cell signature gene. b t-SNE plot of HNSCC scRNA-seq cohort (n = 15), colored by sample groups. c Volcano plot showing differentially expressed genes between B cells activation group and the B cells inhibition group B cells. Down, down regulated DEGs. NoSignifi, not significant. Up, up regulated DEGs. d GO cluster plot showing a chord dendrogram of the clustering of the expression spectrum of significantly upregulated genes in B cells based on the B-cell signature genes classification. e Violin plot showing expression of IGHG1, IGHA1, CD24 and IGHM in B cells activation group and the B cells inhibition group