Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 10;16(4):870–884. doi: 10.1038/s44321-024-00048-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Graph of time to matrix detachment in RDEB fibroblast populations comparing vehicle control (n = 13, 5 biological replicates in 2–3 technical replicates) to 1 µM idoxuridine (n = 6, 5 biological replicates in 1–2 technical replicates) and daclatasvir (n = 6, 5 biological replicates in 1–2 technical replicates) treatment. Kruskal–Wallis test with Dunn’s correction performed for significance. (B) Line graph of RDEB cell proliferation (mean $\pm$ SEM) as a percentage compared to Day 1 with vehicle control (n = 10, 5 biological replicates in duplicate), idoxuridine (n = 10, 5 biological replicates in duplicate), and daclatasvir (n = 10, 5 biological replicates in duplicate). Ordinary one-way ANOVA with Dunnett test performed. (C) Western blots of collagen I, p-AKT, p-SMAD3, and GAPDH in RDEB fibroblasts with vehicle control, exogenous TGFβ, SB431542, idoxuridine, and daclatasvir treatment (left) and quantification of blots presented as graphs of collagen I (n = 5 biological replicates), p-AKT (n = 7 biological replicates), and p-SMAD3 (n = 5 biological replicates) relative to GAPDH. RM one-way ANOVA with Holm–Šídák test for p-AKT and p-SMAD3 and Dunnett test for collagen I. (D) qPCR result of COL1A1 mRNA presented as graph of Fold Change relative to GAPDH mRNA in RDEB fibroblasts (n = 5 biological replicates) with vehicle control, idoxuridine, and daclatasvir. RM one-way ANOVA with Holm–Šídák test for significance. (E) Graph of total hydroxyproline amount (mean $\pm$ SEM) in cell layer and media of RDEB (n = 6 biological replicates), RDEB + idoxuridine (n = 6 biological replicates), RDEB + Daclatasvir (n = 6 biological replicates), non-EB (n = 6 biological replicates), EB + idoxuridine (n = 6 biological replicates), and non-EB + Daclatasvir (n = 6 biological replicates) treatment. (F) Graph of elastic modulus of RDEB matrix with vehicle control (n = 36, 3 biological replicates in 12 technical replicates), idoxuridine (n = 37, 3 biological replicates in 12 technical replicates), and daclatasvir (n = 36, 3 biological replicates in 12 technical replicates) treatment. Kruskal–Wallis test with Dunn’s correction performed for significance. Data information: In (A, C, D, F), data are presented as mean $\pm$ SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, P > 0.05 is not significant (ns). Source data are available online for this figure.