Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 19;16(4):805–822. doi: 10.1038/s44321-024-00053-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Untransfected ARPE-19 cells (control) were labeled for FAM161A (green) and centrin (red) with arrowheads indicating co-labeled centrioles. To validate both HS and HL FAM161A cDNAs, ARPE-19 cells were transfected with the HL or HS isoforms and labeled for FAM161A (green). The labeling of overexpressed HS and HL was strong and needed a 20-fold decrease in exposure time compared to the labeling of endogenous FAM161A in untransfected cells. (B) Western blot of ARPE-19 and 661 W cells nontransfected (ctrl) or transfected with expression plasmids for GFP, HL, or HS showed the different sizes of the two protein isoforms. (C) hTERT-RPE1 cells were transfected with either the HL or HS isoforms and labeled for FAM161A (green) and acetylated tubulin (red). Overexpression of both isoforms colocalize with acetylated tubulin. (D) Schematic representation of the different elements combined in the AAV8 vectors produced for in vivo studies. Three versions of regulatory elements were followed by either of the three types of transgene elements. (E–G) GFP expression (arrows) one-month post subretinal injection of AAV2/8-IRBP-GRK1-, FCBR1-F0.4-, and FCBR2-F0.4-GFP vectors. Scale bar in (A, C) 25 μm and (E) 100 μm. HS human short isoform, HL human long isoform, Ac-Tub acetylated Tubulin, ONL outer nuclear layer, INL inner nuclear layer, RPE retinal pigment epithelium. Data information: Results in (A) are representatives of four (ARPE-19) and two (661 W) independent experiments. One sample for each condition and each experiment was processed for immunocytochemistry. Data obtained in (C) were generated from one single experiment. Results in (E–G) are representative of 5, 12, and 5 experiments, respectively, with the corresponding eye number processed for IHC: n = 12, 6, and 9. Source data are available online for this figure.