Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 21;16(4):723–754. doi: 10.1038/s44321-024-00057-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV2 — (A) Plasma was collected from six PyLARC2 immunized SW mice and pooled. The pooled plasma was then diluted 1:100 and used to evaluate reactivity to antigens at different timepoints of PyWT LS development using IFA. Sera is shown in red, anti-mHSP70 antibody is used as control (green) and DNA is stained with DAPI (blue). Scale bar is 20 µm. At early timepoints of LS development, the immune sera from PyLARC2 immunized mice reacted with antigens expressed on the PVM or PPM. In more mature LS, both cytoplasmic and membrane reactivity was observed, indicating that the serum antibodies recognized antigens located in both the cytoplasm and the membranes of the parasite. No reactivity was observed in plasma harvested from mock-immunized mice, indicating that the staining was specific to the PyLARC2 immunized mice. (B) Plasma was collected from six PyLARC2 immunized SW mice or BALB/cJ mice. Pooled plasma for each mouse strain was diluted 1:100 and used to evaluate reactivity to antigens expressed in PyWT blood stages. Sera is shown in red, anti-MSP1antibody is used as control (green) and DNA is stained with DAPI (blue). Scale bar is 10 µm. Immune sera from both SW and BALB/cJ mice reacted with antigens expressed in the cytoplasm of blood stages. No reactivity was observed in plasma harvested from mock-immunized mice, indicating that the staining was specific to immune sera from PyLARC2 immunized mice.