FIGURE 5.

Forced FRAIL1 expression alters the global skeletal muscle proteome and transcriptome. (a, b) One TA muscle per mouse (n = 5) was transfected with 10 μg of empty plasmid (pcDNA3), and the contralateral TA in each mouse was transfected with 10 μg of full‐length FRAIL1 expressing plasmid. Seven days posttransfection, bilateral TAs were harvested and subjected to TMT‐mass spectrometry. In a separate cohort, one TA muscle per mouse (n = 6) was transfected with 10 μg of empty plasmid (pcDNA3), and the contralateral TA in each mouse was transfected with 10 μg of full‐length FRAIL1 expressing plasmid. Seven days posttransfection, bilateral TAs were harvested and subjected to RNA‐seq analysis. (a, b) Volcano plots of differences in global protein (a) and transcript (b) abundance following ectopic FRAIL1 expression. (c, d) Heatmaps of key skeletal muscle proteins (c) and mRNAs (d) differentially expressed in response to FRAIL1 expression. False discovery rate (FDR) was determined by Benjamini‐Hochberg correction. * FDR ≤ 0.05, ** FDR ≤ 0.01.