FIGURE 7.

Forced FRAIL1 expression downregulates transcripts and proteins involved in the regulation of skeletal muscle structure, energy production, and metabolism. Gene set enrichment analysis (GSEA) of RNA‐seq data was used to identify pathways that were repressed by FRAIL1 expression in mouse skeletal muscle. (a, b) Enrichment plots of the top five Reactome gene sets that were repressed by FRAIL1 expression in mouse tibialis anterior muscles (a) and heatmap of key individual transcripts from those gene sets (b) downregulated in response to FRAIL1. Colored dots left of heatmaps indicate the gene set(s) of origin for the associated gene. (c, d) Enrichment plots of overrepresented cellular component GO terms among skeletal muscle proteins decreased by ectopic FRAIL1 expression (c) and key downregulated proteins from those ontologies (d). False discovery rate (FDR) was determined by Benjamini‐Hochberg correction. *FDR ≤ 0.05, **FDR ≤ 0.01.