Figure 4.

Luminescence emitted from F12^lacZ parasites remains stable. (A) Chemiluminescence emitted by F12^lacZ parasites synchronized to different stages of intraerythrocytic development. Linear regression was calculated using values significantly different from the F12^wt ctrl. (B) Following addition of the β-gal^SENSOR probe, luminescence remains stable over a wide range of parasite numbers and stages for at least 180 min. Absolute parasite numbers used per condition are indicated. 20 000 wild type parasites (F12^wt) were used as negative controls (ctrl.). For (A), n = 3; biological replicates are shown individually; for the control samples (ctrl.), values were averaged, and error bars indicate standard deviations of biological replicates. For (B), a representative example of three biological replicates is shown. The full data set is shown in supplementary Figure 3.