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. 1998 Oct;72(10):8309–8315. doi: 10.1128/jvi.72.10.8309-8315.1998

FIG. 5.

FIG. 5

S1 nuclease protection analysis of the KSHV latency promoter. Shown is an autoradiogram of a 12% denaturing acrylamide gel. Lane 1 shows the input, undigested probe; lanes 2 to 4 show the protected fragments resulting from hybridization to 30 μg of BCBL RNA (lane 2), 15 μg of BCBL-1 RNA (lane 3), or 30 μg of yeast RNA (lane 4) followed by S1 nuclease digestion at 28°C. The arrows point to the two most prominent protected fragments, and size standards are indicated on the left. The asterisks denote the site of the radiolabel.