Fig. 1.

Signal crosstalk in two-photon all-optical physiology system. (a) Typical image of mouse hippocampus CA1 in vivo. jGCaMP8m is labeled for calcium imaging. mCherry and ChRmine are co-labeled for two-photon photostimulation. Scale bar: 80 µm. (b) Up: Emission spectra of typical red indicators. Data from [12]. Green range indicates the green filter range (500 nm ∼ 550 nm) of our two-photon all-optical physiology system. Down: Two-photon excitation spectrum for GCaMP6m. Data from [13]. Red vertical line indicates the wavelength (1040 nm) of two-photon photostimulation beam. (c) Typical images of recording channel before (t = 0 ms), during (t = 17∼51 ms) and after (t = 68, 85 ms) photostimulation. Scale bar: 80 µm.