TABLE 1.
Transgene constructa | No. of lines testedb | No. of transgenic linesc complementing mutant:
|
|||
---|---|---|---|---|---|
Δ25K | Δ12K | 8K+6 | |||
TGB100 | 22 | 16 | —d | — | |
TGB200 | 9 | — | 3 | — | |
TGB300 | 11 | — | — | 7 | |
TGB400 | 21 | 21 | 0e | 0 | |
TGB500 | 5 | 5 | 0 | — | |
TGB600 | 6 | — | 4 | 3 |
Dark boxes indicate promoter and terminator regions. 25K, 12K, and 8K are the names of the TGB ORFs of PVX.
Ten to 20 plants per line were tested by inoculation with each virus.
Number of lines complementing cell-to-cell movement of mutant viruses was measured by GUS assay at 5 dpi. Wild-type PVX.GUS moved from cell to cell in all lines tested.
—, lines not tested with specific viruses.
Lines yielding no positive results.