TABLE 1.

Single gene E. coli BW25113 Keio knockouts validated in this study

Predicted mutant phenotype	Gene	Function	TraDIS log2FC		Experimental mutant phenotype MIC50 (ng/mL)
			Before ChromoCorrect	After ChromoCorrect
-	WT	-	-	-	10
Cip sensitive before and after	acrB	Multidrug efflux system protein	−0.89	−0.83	≤5
	recA	DNA recombination and repair protein	−1.99	−1.92	≤5
Cip resistant before and WT phenotype after	cyaA	Adenylate cyclase	2.48	0.15	10
	ilvN	Acetolactate synthase 1 small subunit	2.13	−0.06	10
	mtlD	Mannitol-1-phosphate dehydrogenase	1.77	0.13	10
	fdhE	Formate dehydrogenase formation protein	1.66	0.13	10
	pfkA	6-phosphofructokinase I	1.32	−0.03	10
Cip resistant before and after	purH	Bifunctional transformylase/ cyclohydrolase	2.50	1.64	20
	dgkA	Diacylglycerol kinase	1.62	1.07	20
	rpe	D-ribulose-5-phosphate 3-epimerase	1.45	1.07	20
Cip resistant before and sensitive after	uvrD	DNA-dependent ATPase I and helicase II	1.32	−1.06	≤5