Figure 3:

(A) LKB1 expression is downregulated in donor lungs compared to recipient lungs at day 34. Representative immunohistochemistry of LKB1 expression in donor and recipient lung tissue at day 34 after LTx. (B) STE20-related adaptor alpha (STRADα, LKB1, pAMPK expression is downregulated and pmTOR expression is upregulated in donor lungs compared to recipient lungs at day 34, as detected by western blot analysis. (C, D, E, F) Densitometry analysis showed significant downregulation of STRADα (n=3, p=0.002), LKB1 (n=3, p=0.013), and pAMPK (n=3, p=0.01) and upregulation of pmTOR (n=3, p=0.006). Values are mean ± SD of three independent experiments. Abbreviations: AMPK, AMP-activated protein kinase; mTOR, mammalian target of rapamycin.