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Tracings of intracellular Ca2+ flux in cells expressing US28 after stimulation with chemokines. Each tracing is representative of three separate runs. (A) Single stimulation of 293 and 293.Gα16 cells expressing US28 with RANTES, MCP-3, and MIP-1α. (B) Sequential stimulation of 293 and 293.Gα16 cells expressing US28 with either 1 or 10 nM RANTES (R) or 10 nM MCP-3 (M).
