Fig 6.

Knockdown of ACE2 suppresses multicycle replication of MERS-CoV and cleavage of S in Calu-3 airway cells. (A) Calu-3 cells were treated with ACE2-AUG PPMO or remained untreated (w/o) for 24 h. Cells were then inoculated with MERS-CoV at a MOI of 0.001. Virus titers in supernatants were determined by TCID50 at indicated time points post-infection. The mean + SD of three independent experiments are shown. (B) Calu-3 cells treated with ACE2-AUG or scramble PPMO or without PPMO treatment were infected with MERS-CoV as described above. At 72 h p.i., cell lysates were subjected to SDS-PAGE and western blot analysis using MERS-CoV S2-specific antibodies. Uninfected cells served as control (mock).