TABLE 1.
Sequences of primers and probes used in RT-PCR and Southern blot hybridization
| Transcript | Product size (bp) | Sequence (5′–3′) and type of primer |
|---|---|---|
| EBNA1a | 273 | GATGAGCGTTTGGGAGAGCTGATTCTGCA (Sense) |
| TCCTCGTCCATGGTTATCAC (Antisense) | ||
| AGACCTGGGAGCAGATTCAC (Probe) | ||
| EBNA2a | 339 | GCTGCTACGCATTAGAGACC (Sense) |
| TCCTGGTAGGGATTCGAGGG (Antisense) | ||
| CAGCACTGGCGTGTGACGTGGTGTAAAGTT (Probe) | ||
| Qp initiateda | 339 | AGGCGCGGGATAGCGTGCGCTACCGGA (Sense) |
| TCCTCGTCCATGGTTATCAC (Antisense) | ||
| AGACCTGGGAGCAGATTCAC (Probe) | ||
| Cp initiateda | 297 | CACTACAAGACCTACGCCTCTCCATCCATC (Sense) |
| TCTCCCCTAGGCCCTGAAGGTGAACCGCTT (Antisense) | ||
| GCGACCGGTGCCTTCTTAGGAGCTGTCCGA (Probe) | ||
| Wp initiateda | 235 | TCAGAGCGCCAGGAGTCCACACAAAT (Sense) |
| TCTCCCCTAGGCCCTGAAGGTGAACCGCTT (Antisense) | ||
| GCGACCGGTGCCTTCTTAGGAGCTGTCCGA (Probe) | ||
| LMP1a | 490 | TCCTCCTCTTGGCGCTACTG (Sense) |
| TCATCACTGTGTCGTTGTCC (Antisense) | ||
| GAAAGCACAATTCCAAGGAACAATGCCTG (Probe) | ||
| LMP2Ab | 280 | ATGACTCATCTCAACACATA (Sense) |
| CATGTTAGGCAAATTGCAAA (Antisense) | ||
| ATCCAGTATGCCTGCCTGTA (Probe) | ||
| LMP2Bb | 325 | CAGTGTAATCTGCACAAAGA (Sense) |
| CATGTTAGGCAAATTGCAAA (Antisense) | ||
| ATCCAGTATGCCTGCCTGTA (Probe) | ||
| BZLF1a | 453 | CATGTTTCAACCGCTCCGACTGG (Sense) |
| GCGCAGCCTGTCATTTTCAGATG (Antisense) | ||
| GCACGACGCACACGGAAACCACAACAGCCA (Probe) |