TABLE 2.
MT2 cDNA library clones promoting β-galactosidase activity in yeast cotransformed with pGBT-Tax
| GenBank match (accession no.) | No. of positive clones | β-Galactosidase filter assay resulta
|
||
|---|---|---|---|---|
| pGBT Tax | pGBT9 | pGBT Lamin | ||
| Tax protein (S67443) | 31 | +++ | − | − |
| Human CREB-2 mRNA (M86842) | 29 | +++ | − | − |
| Human homolog of Drosophila discs large tumor suppressor (U13897) | 28 | +++ | − | − |
| Human myeloblast KIAA0147 gene (D63481) | 27 | +++ | − | − |
| Human Tax-binding protein TXBP151 mRNA (U33821) | 24 | +++ | − | − |
| Hexapeptide TSDGLC | 11 | +++ | − | − |
| No match | 8 | +++ | − | − |
a
Following plasmid isolation in HB101 bacteria, pGAD cDNA was reintroduced into the HF7c yeast strain with the indicated plasmids. Four days after transformation, the transformants were subjected to galactosidase filter assay. Symbols: +++, dark blue colonies that turned blue after 0.5 h; −, colonies that were still white after 8 h.