Fig. 2.

Demultiplexing and Doublet Detecting Method Performance Comparison. a The proportion of droplets classified as singlets and doublets by each method in the PBMCs. b The number of other methods that classified the singlets and doublets identified by each method in the PBMCs. c The proportion of droplets classified as singlets and doublets by each method in the fibroblasts. d The number of other methods that classified the singlets and doublets identified by each method in the fibroblasts. e-f The performance of each method when the majority classification of each droplet is considered the correct annotation in the PBMCs (e) and fibroblasts (f). g-h The number of droplets classified as singlets (box plots) and doublets (bar plots) by all methods in the PBMC (g) and fibroblast (h) pools. i-j The number of donors that were not identified by each method in each pool for PBMCs (i) and fibroblasts (j). PBMC: peripheral blood mononuclear cell. MCC: Matthew’s correlationcoefficient