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. 2024 Apr 15;25:94. doi: 10.1186/s13059-024-03224-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Recommended Method Combinations Dependent on Experimental Design. Method combinations are provided for different experimental designs, including those that are not multiplexed (left) and multiplexed (right), including experiments that have reference SNP genotypes available vs those that do not and finally, multiplexed experiments with different numbers of individuals multiplexed. The each bar represents either a single method (shown with the coloured icon above the bar) or a combination of methods (shown with the addition of the methods and an arrow indicating the bar). The proportion of true singlets, true doublets, false singlets and false doublets for each method or combination of methods is shown with the filled barplot and the MCC is shown with the black points overlaid on the barplot. MCC: Matthew’s Correlation Coefficient