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. 2024 Apr 16;16:84. doi: 10.1186/s13098-024-01332-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Overexpression of apelin in WJ-MSCs and analysis of transduction efficiency. (a) Immunofluorescence images showing the intracellular GFP fluorescence in AP-WJ-MSCs (Blue: nuclear DAPI, Red: Apelin, Green: GFP). (b) Quantitative flow cytometric analysis confirming the efficiency of lentiviral transduction in WJ-MSCs. (c) qRT-PCR results indicating a 36.74-fold increase in Apelin gene expression in Ap-MSC-sEVs compared to that in MSC-sEVs (***P < 0.001). (d–e) Western blot images depicting the protein expression levels of apelin in AP-MSC-sEVs (***P < 0.001). Abbreviations: sEVs, small extracellular vesicles; AP-MSC-sEVs, apelin-MSC-sEVs, engineered sEVs loaded with overexpressed apelin; Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs)