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Glycosylation of ACP-bound AHBA and subsequent modification in mitomycin biosynthesis. (a) attachment of AHBA to the acyl carrier protein MmcB and glycosylation by the glycosyltransferase MitB; (b) deacetylation of GlcNAc-AHBA-MmcB to GlcN-AHBA-MmcB and transformation of the sugar moiety to a linear aminodiol that terminates with an epoxyethane catalyzed by the NADPH-dependent protein MitF and the radical SAM protein MitD.
