Extended Data Fig. 6. Gastrulation is not affected by changes in hydrostatic pressure.
a, Schematic of mechanically changing blastocoel volume. b. In situ hybridization of embryos at stage 10 analyzing the pan-mesodermal markers Xbra and the neural crest inducer gene Wnt8. c, Percentage of embryos expressing mesodermal markers comparing control to inflated and deflated embryos. d, In situ hybridization of embryos at stage 14 analyzing the pan-mesodermal marker Xbra and neural crest marker gene Wnt8. e, Spread of data points indicating the change in notochord length normalized to total length in control (n = 7 embryos), inflation (n = 16 embryos), and deflation (n = 13 embryos). f, Effect of inflation and deflation on mesoderm ability to produce Wnt8. g, Spread of data points comparing change in Wnt8 expression in control (n = 8 embryos), inflation (n = 8 embryos), and deflation (n = 8 embryos). h, Blastopore formation and closure after inflation and deflation compared to control. i, Diameter of blastopore closure in control (n = 9 embryos), inflation (n = 8 embryos), and deflation (n = 9 embryos). Scale bar 350 µm (b, d, f, and h). Data (e, g, and i) represent mean, error bars are s.d. Statistical analysis was performed using two-sided Dunnett’s test (NS, P > 0.05 e, g, and i, CI = 95%). Three independent experiments (b and d).