Fig. 3. Hydrostatic pressure controls neural crest competence.
a–c, Schematic of inflating embryo with hypotonic (a), hypertonic (b) and blastocoel fluid (c). d, Spread of data points comparing the change in snai2 expression at stage 14. e, Schematic of reinflating an embryo to normal blastocoel size after deflation with a saline solution. f, Spread of data points comparing changes in snai2 expression at stage 14. g,i, Left to right: the schematic of DLMZ graft assay into stage 12 and 10 embryos, respectively and analysed via ISH for snai2 at stages 18 and 16, respectively. h,j, Quantification of neural crest competence assay during deflation (h) and inflation (j) of Xenopus embryos. Scale bar, 450 µm (g,i). Data are mean and s.d. Statistical analysis was performed using two-sided Dunn’s test and unpaired t-tests (NS, P = 0.2590 (f), ***P = 0.0001 (d), ****P ≤ 0.0001 (d,f,h,j), 95% CI). n = 9control, 10hypotonic, 8hypertonic and 13blastocoel embryos (d), n = 10control, 15deflation and 23reinflation embryos (f), ncompetence = 16control and deflation embryos (h), ncompetence = 18control and inflation embryos (j).