Fig. 7. Nuclear Yap and β-catenin extends neural crest competence.
a, Immunofluorescence of iNCCs at different confluency stained for β-catenin and Yap. b, Quantification of Yap nuclear-to-cytoplasmic ratio. c, Pearson’s coefficient and overlap of β-catenin and Yap at different confluencies. d, Co-immunoprecipitation analysis of β-catenin, no specific binding IgG. e, Schematic of neural crest competence assay and in situ hybridization analysis of snai2 at stage 18. f, Quantification of neural crest competence normalized to control. Scale bars, 20 µm (a) and 400 µm (e). Data are mean and s.d. Statistical analysis was performed using ordinary two-way ANOVA and two-sided unpaired t-test (**P ≤ 0.0058 (b), ****P = 0.0001 (b,f), 95% CI). Three independent experiments (b,c). n = 11control, 12Yap(S127A) embryos (f). Three independent experiments (d). An unprocessed blot is available as Source data.