Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 18;26(4):530–541. doi: 10.1038/s41556-024-01378-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 2 — a, Quantification of embryo volume at stage 10 (n = 18 embryos), 11 (n = 18 embryos), and 12 (n = 18 embryos). b, Percentage of the ratio of blastocoel volume to whole embryo volume at stage 10 (n = 17), 11 (n = 18), and 12 (n = 12). c, Schematic of embryos at different stages showing microelectrode penetration (0.5-1 µm tip) through ectoderm. d, Profile of a successful measurement of blastocoel hydrostatic pressure. Pressure is close to zero whilst the microelectrode is not in contact with the embryo before and after measurement. A transitory spike is noted during penetration of the microelectrode through the ectoderm. The spike is followed by a stable phase between five to ten seconds, indicating the actual blastocoel pressure. e, Correlation between Hydrostatic Pressure and Competence. ‘R² = 0.99 and beta = −7.7, as calculated using orthogonal distance regression (ODR) to account for measurement variability in estimating neural crest competence and the hydrostatic pressure within the blastocoel cavity. Statistical analysis was performed using two-sided Dunnett’s test (NS, P > 0.1639; a, ***P = 0.0003; b, ****P ≤ 0.0001; b, CI = 95%). Box plots (a and b) show median, 25^th and 75^th percentiles, and whiskers extend to minimum and maximum values. Data represent mean, error bars are s.d. (e). Three independent experiments (e).

Source data