FIGURE 4.

Effect of angiotensin II, cholecalciferol, and calcitriol on FoxO3a protein expression in C2C12 myotubes. (a) Representative FoxO3a protein expression on Day 7 of the differentiation phase evaluated by western blotting. (b) Quantification of FoxO3a protein expression. The band density was normalized to that of GAPDH (n = 7/group, independent experiments). The data were analyzed using one‐way ANOVA with Tukey's post hoc test. Angiotensin II (AII), cholecalciferol (VD₃), calcitriol (1,25VD₃), and losartan (Lo) were used.