FIGURE 6.

Effect of angiotensin II, cholecalciferol, and calcitriol on the expression of autophagy‐lysosomal‐related proteins in C2C12 myotubes. (a) Representative images of LC3B‐I, LC3B‐II, p62/SQSTM1, and cathepsin. (b–g) Quantitative analysis of the LC3B‐II/LC3B‐I ratio, LC3B‐II, LC3B‐I, p62/SQSTM1, mature cathepsin L, and pro‐cathepsin L protein expression. LC3B‐II, LC3B‐I, p62/SQSTM1, mature, and pro‐ cathepsin L expression was normalized to that of GAPDH (n = 7/group, independent experiments). The data were analyzed using the Kruskal–Wallis test with Dunn's test (LC3B‐II/LC3B‐I ratio, LC3B‐II, and p62/SQSTM1) and one‐way ANOVA with Tukey's post hoc test (LC3B‐I, mature and pro cathepsin L). Angiotensin II (AII), cholecalciferol (VD₃), calcitriol (1,25VD₃), and losartan (Lo) were used.