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Stringent control of reporter gene expression. HeLa cells were coinfected with 10 PFU of the respective activator and reporter viruses. The inducer (4 μM) was added at the start of infection, and cell extracts were prepared at 16 h postinfection. CAT protein expression was quantitated by using a CAT ELISA kit (Boehringer Mannheim). The mean value of CAT protein expression, calculated from seven independent experiments, is shown.
