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. 2023 Dec 19;514(1):1–5. doi: 10.1134/S1607672923700576

Fig. 1.

Fig. 1.

Tricistronic expression plasmids encoding glycoprotein hormone chains and signal peptide sequences. (a) Plasmid scheme, designations: pUC origin, region of origin of replication of the pUC plasmid; bla, beta-lactamase open reading frame; EBVTR, human Epstein–Barr virus terminal repeat region; CHO EEF1A1 UFR and CHO EEF1A1 DFR, regions flanking the Chinese hamster EEF1A1 gene, contain the promoter, intron, terminator, and polyadenylation signal of the EEF1A1 gene; TATA, TATA box; PTS, putative transcription start; IRES, natural internal ribosome entry site of wild-type EMCV; IRESatt, attenuated internal ribosome entry site; *SP, β-chain variable signal peptide; β, β-chain ORF; αSP, signal peptide of the glycoprotein hormone α-chain; α, α-chain ORF; DHFR, mouse dihydrofolate reductase ORF. (b) Sequences of signal peptides, the N-region is highlighted in purple according to the SignalP algorithm, the H-region is in blue, and the C-region is in orange.