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. 2023 Dec 19;514(1):1–5. doi: 10.1134/S1607672923700576

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© The Author(s) 2024, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2024, Vol. 514, pp. 1–5. © The Author(s), 2024. This article is an open access publication.ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2023. © The Author(s), 2023. This article is an open access publication.

Open Access.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2. — Titer of glycoprotein hormones secreted by a transiently transfected CHO cell culture when varying the signal peptides of their β-chains. (a–d) Titer of the heterodimeric form of the hormone (assessed by ELISA) normalized to the level of the control green fluorescent protein eGFP (assessed by fluorimetry). (e–h) Titer of the secreted α-chain with the native signal peptide (assessed by ELISA) normalized to the titer of the heterodimeric form of the hormone. Data are represented as the mean value of three independent biological replicates, with two ELISA measurements for each sample; the value for the control peptide NSP is taken as 100%. * p < 0.05, Tukey’s test. Abbreviations: FSH, follicle-stimulating hormone; LH, luteinizing hormone; hCG, human chorionic gonadotropin; TSH, thyroid-stimulating hormone; NSP, native signal peptide of the corresponding glycoprotein hormone β-chain; HSA, human serum albumin; Azu, azurocidin; aSP, signal peptide of the glycoprotein hormone α-chain.