Table 2.

Number of protein-coding DEGs identified by differential expression analysis in CTL and ALK1-mutated ECFCs and HMVECs

Comparison	ECFCs			HMVECs
	Total DEGs	Upregulated	Downregulated	Total DEGs	Upregulated	Downregulated
CTL NS vs B9	828	456	372	704	366	338
CTL NS vs B10	787	418	369	481	225	256
CTL B9 vs B10	0	0	0	2	0	2
MUT NS vs B9	604	310	294	295	158	137
MUT NS vs B10	564	278	286	206	90	116
MUT B9 vs B10	0	0	0	0	0	0
CTL NS vs MUT NS	28	11	17	1261	528	733
CTL B9 vs MUT B9	19	6	13	1262	502	760
CTL B10 vs MUT B10	30	7	23	1164	503	661

3 CTL ECFCs and 2 ALK1-mutated ECFCs (MUT-H1–H2) or 3 CTL HMVECs and 2 ALK1-mutated HMVECs (MUT-P1–P2) were stimulated or not with BMP9 or BMP10 (10 ng/mL) for 18 h. The experiment was repeated three times to generate technical replicates, after which bulk RNA-seq analysis was performed using DESeq2 package with an absolute log₂ fold change threshold of 1 (llog₂FCl ≥ 1) and a adjusted p-value ≤ 0.05 (Benjamini–Hochberg procedure). Data show protein coding DEGS analyzed by NS non-stimulated, B9 BMP9-stimulated, B10 BMP10-stimulated