Figure 5.

Alexidine analogs inhibit cell invasion

(A) H1299 cells were embedded as spheroids in recombinant basement membrane matrix and treated with the indicated analogs for 48 h. Representative images of cell invasion are shown. The borders of cell invasion are outlined in white. Scale bar = 250 μm.

(B) Quantification of invasive area of cells treated as in (A) and normalized to DMSO control.

(C) Cells were treated for 48 h with the analogs, as indicated, in 2D cell culture. Lysates were separated by western blot and probed for phosphorylated FAK (pFAK (Y397)) and total FAK. Actin was used as a loading control. See Figure S6 for quantification of the western blots. For all experiments, three biological replicates were performed. For (A) and (B), at least four spheroids per treatment were assessed for each biological replicate. Mean ± SD is shown. Significance was assessed using an ordinary two-way ANOVA with a Tukey’s multiple comparisons test, ∗p ≤ 0.05, ∗∗p ≤ 0.01.