Tris-Buffered Saline with 0.1% Tween 20 (TBST) 1× Solution

Reagent	Final concentration	Amount
10× TBS Stock Solution	1×	100 mL
Tween 20 Detergent	0.1% w/v	1 mL
ddH2O	N/A	Adjust to final volume of 1 L (∼900 mL)
Total	1×

Store at 4$°C$ for 3 months.