FIG. 3.

(A) Southern blot analysis of human genomic DNA with a probe specific for the HERV-K-T47D LTR (Fig. 1, probe 1). DNA samples (10 μg/lane) from healthy individuals (1 to 5) were restricted to completion, blotted, and hybridized under relaxed stringency conditions (5× SSC, 60°C). Restriction enzymes are abbreviated as follows: B, BamHI; E, EcoRI; and H, HindIII. Marker sizes are indicated on the left. (B) Southern blot analysis of DNA derived from Old World and New World monkeys and higher primates, using a probe specific for the HERV-K-T47D pol gene (Fig. 1, probe 2). High-molecular-weight DNA (10 μg/lane) was restricted to completion with HindIII, blotted, and hybridized under relaxed stringency conditions. The DNAs analyzed were as follows: lane 1, human; lane 2, chimpanzee; lane 3, orangutan; lane 4, Presbytis; lane 5, baboon; lane 6, rhesus monkey; and lane 7, Aotes.