FIG. 2.

Concentrations of Rosco and Olo required to inhibit HSV-1 replication in HEL and Vero cells. HEL or Vero cells (2 × 10⁵) in 12-well plates (500 cells/mm²) were infected with 2.5 PFU of HSV-1 strain KOS per cell. One hour later, the inoculum was removed, the cells were washed twice with cold PBS, and medium containing the indicated concentrations of Rosco (A), Olo (B), or PAA (C) was added. Cultures treated with Olo required a medium change at 6 hpi. After 24 h, cells were harvested and virus was titrated by standard plaque assay. Viral titers at 24 hpi are plotted against the drug concentration. Note that the scales on the y axis differ between panel B and panels A and C. Each time point indicates the average and range of two experiments.